The key result came at the Memorial Ground, where Bristol Rovers beat Swansea 2-1 - meaning the Iron opened up a two point gap ahead of the Swans, with two games left to play.
Southend also lost their grip on the top of the table, as they lost at Oxford.
It allowed Yeovil to reclaim the top spot, with Lee Johnson's strike earning the Glovers a vital point at home to Wycombe.
Cambridge were relegated to the Conference after their goalless draw against Rochdale. Hundreds of fans satges a sit-down protest after the game at the Abbey Stadium.
Kidderminster will follow them out of the Football League next week when they host Grimsby.
They lost 3-0 at Boston and could mathematically stay up - but they would need to win both their remaining games, hope Rushden lose both of theirs, and overcome a staggeringly inferior goal difference.
It is not going to happen - and their spell in the League will end when they host the Cods next week.
Bury recorded a comfortable 3-1 victory with Cheltenham at Gigg Lane. Brian Barry-Murphy, Colin Kazim-Richards and Ricky Shakes scored in the first half for the hosts, with Stephen Gillespie replying from the spot for the Robins.
Darlington look set for the play-offs after two penalties from Clyde Wijnhard gave them a home victory over Rushden.
Macclesfield are also on course for a top-seven finish after their 3-1 win against Mansfield at Moss Rose.
Leyton Orient comfortably beat nine-man Chester 2-0 at Brisbane Road.
Josh Low scored the winner for promotion-chasing Northampton in their 1-0 win over Lincoln at the Sixfields Stadium.
Shrewsbury and Notts County drew 1-1 at Gay Meadow.