Rovers moved back into the play-off positions with a 1-0 win over Crewe on Tuesday night.
And while three points would not be enough to move Tranmere up any league positions, it would increase the pressure on the four teams above them ahead of Saturday's fixtures.
"We've got to win. it's as simple as that," declared Moore.
"We've got ourselves in a nice position and we need to take advantage.
"Everybody is fit. Chris Greenacre has suffered with the flu, and I'm not quite sure whether he'll be able to do the full 90 minutes, but apart from that everybody is fine."