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A Scunthorpe side including Cliff Byrne, Adam Capp, Tommy Johnson, Wayne Corden, Andy Parton and Lee Ridley (making his comeback from a hernia problem that has kept him out so far this season) had their chances, with Parton and Corden going close in particular.
Corden had a rasping 30-yard drive saved, whilst Parton came close on a couple of occasions.
With the score at 2-0, Parton also missed a penalty after he was brought down in the Doncaster box.
A positive though was the return to action of Ridley, who played 75 minutes of the game.
It was a disappointing result which leaves the reserves without a win, three games into the new season, and Iron boss Brian Laws will be hoping the first team can avenge this defeat on Saturday.
Teams:
Doncaster: Budtz, Marples (Ireland 65), Nelthorpe, S Roberts, Fenton, Hughes, Coppinger, Griffiths, N Roberts (c), Heffernan, Offiong (Saunders 80).
Scunthorpe: Capp, Byrne, Ridley (Winn 75), Mazzola, Smith, Twibey, McGhie (Fox 53), Mitchell, Johnson, Parton, Corden.