Tuesday night's game will see the left-back Marcus Williams make his first appearance of pre-season, after being omitted from the squads for the two games so far while his new two-year contract was being finalised.
United have released all the trialists who featured against Brigg and Doncaster apart from the left-back Michael Lea, who is likely to be on the bench at the Don Valley Stadium with Williams handed a starting spot.
That means the manager Nigel Adkins has a much more streamlined squad, and is working with the same players he will have to choose from when the season gets underway against Leeds United on Saturday August 9.