The Iron will head to Northolme to take on Blue Square North side Trinity on Tuesday July 19.
They will start their defence of the Lincolnshire Senior Cup five days later, on Sunday July 24, when they head to Sincil Bank.
The match is a semi-final in the competition, with the winner of the tie then heading to Bundell Park to face Grimsby Town in the final.
Scunthorpe, the only Football League team in Lincolnshire following the relegation of Grimsby and Lincoln into the Blue Square Premier, have won the competition in the last four seasons.