Sixty teams will be competing to reach the final in the Millennium Stadium, including twelve teams from the Nationwide Conference.
Four teams have been handed byes to the second round, with Bury and Huddersfield the two teams from the northern section.
Northern Section
1. Barnsley 2. Blackpool 3. Carlisle United 4. Chester City 5. Chesterfield 6. Darlington 7. Doncaster Rovers 8. Grimsby Town 9. Halifax Town 10. Hartlepool United 11. Hull City 12. Lincoln City 13. Macclesfield Town 14. Mansfield Town 15. Morecambe 16. Notts County 17. Oldham Athletic 18. Port Vale 19. Rochdale 20. Scarborough 21. Scunthorpe United 22. Sheffield Wednesday 23. Shrewsbury Town 24. Stockport County 25. Telford United 26. Tranmere Rovers 27. Wrexham 28. York City
Southern Section
1. AFC Bournemouth 2. Barnet 3. Boston United 4. Brentford 5. Brighton & Hove Albion 6. Bristol City 7. Bristol Rovers 8. Cambridge United 9. Cheltenham Town 10. Colchester United 11. Dagenham & Redbridge 12. Exeter City 13. Forest Green Rovers 14. Hereford United 15. Kidderminster Harriers 16. Leyton Orient 17. Luton Town 18. Oxford United 19. Peterborough United 20. Plymouth Argyle 21. Queens Park Rangers 22. Rushden & Diamonds 23. Southend United 24. Stevenage Borough 25. Swindon Town 26. Torquay United 27. Wycombe Wanderers 28. Yeovil Town.