Hooper has a back injury and is unlikely to be rested against the League One side.
Nigel Adkins, the Iron manager, will also be without David Mirfin and Garry Thompson.
Mirfin is expected to miss the whole of pre-season with an ankle injury, which leaves the centre-back unlikely to be fit for the season opening Championship game at Cardiff City next weekend.
The winger Thompson, preparing to start his second season as an Iron player, has a facial injury.
Saturday's game is pay on the gate, with admission £10 for adults and £5 concessions.