Hooper suffered a dead leg in Friday night's 2-2 draw at Leicester City, and United hope to have him available for the crunch visit of Tranmere Rovers on Saturday.
So long as Scunthorpe avoid defeat on Tuesday night, they will reach the play-offs if they also do not lose to Tranmere.
Andrew Wright is expected to be available to face the Robins despite requiring 10 stitches to a head wound at the Walkers Stadium.
Martyn Woolford's ankle injury is not as bad as first feared, and he could be available for the second leg of a play-off semi-final should Scunthorpe secure a top six finish.