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Iron boss Brian Laws decided to risk playing Crosby after originally toying with the idea of leaving him out and drafting Cliff Byrne into the starting line-up, and virus victim Ian Baraclough onto the bench.
United went on to lose after going into a promising 2-0 lead, with the veteran captain limping through the game.
Laws told the Scunthorpe Telegraph: "We knew there was a risk playing Crozzer, but with so many other players missing, we felt it was one we had to take - and one Andy himself was happy to go along with.
"Hopefully it will just be the Tranmere game that he misses."
Meanwhile, Laws hopes to bring in a loan player before Friday's game.